tcdb peptide library Search Results


tcdb peptide library  (Immune Epitope Database & Analysis Resource)
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Immune Epitope Database & Analysis Resource tcdb peptide library
<t>TcdB</t> 1918 and TcdB 1961 <t>are</t> <t>immunodominant</t> epitopes following vaccination. (A) Amino acid sequences from TcdB peptide library . 15mers were analyzed using the Immune epitope database MHC-II peptide binding prediction for H2-IAb. Data were represented as adjusted rank which is generated by comparing each peptide’s score to a database of 15mers. Lower numbers are predicted to be better binders to H2-IAb. (B) Representative FACS plots showing frequency of TcdB pooled peptide library, individual peptides, or DMSO stimulated CD4 + T cells expressing IFN-γ. (C) The total number of IFN-γ + CD4 + T cells responding to TcdB peptide library or individual peptides. To account for the background cytokine production observed, the total number of IFN-γ + CD4 + T cells in the DMSO group was subtracted from the total number of peptide-responsive IFN-γ + CD4 + T cells. Naïve n=5 and TcdB-CROPs mRNA vaccinated n=5 per timepoint. Two-way ANOVA with Tukey’s multiple comparison test. Statistical significance is indicated as follows: ** P < 0.01; **** P < 0.0001. Representative FACS plots were pre-gated based on the following parameters: Singlets, Live, Lymphocytes, CD45 + , CD3/5 + , CD4 + CD44 + , CD62L lo . Frequency of parental gate +/− standard error of the mean is shown in green. Cells were stimulated with peptide or DMSO for 1 hour before the addition of BFA-M. Cells were incubated for an additional 4 hours before intracellular cytokine staining and analysis by flow cytometry.
Tcdb Peptide Library, supplied by Immune Epitope Database & Analysis Resource, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tcdb+peptide+library/library+peptide+tcdb/bio_rxiv__64898__2026__02__18__706639-34-5-16
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tcdb peptide library - by Bioz Stars, 2026-09
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TcdB 1918 and TcdB 1961 are immunodominant epitopes following vaccination. (A) Amino acid sequences from TcdB peptide library . 15mers were analyzed using the Immune epitope database MHC-II peptide binding prediction for H2-IAb. Data were represented as adjusted rank which is generated by comparing each peptide’s score to a database of 15mers. Lower numbers are predicted to be better binders to H2-IAb. (B) Representative FACS plots showing frequency of TcdB pooled peptide library, individual peptides, or DMSO stimulated CD4 + T cells expressing IFN-γ. (C) The total number of IFN-γ + CD4 + T cells responding to TcdB peptide library or individual peptides. To account for the background cytokine production observed, the total number of IFN-γ + CD4 + T cells in the DMSO group was subtracted from the total number of peptide-responsive IFN-γ + CD4 + T cells. Naïve n=5 and TcdB-CROPs mRNA vaccinated n=5 per timepoint. Two-way ANOVA with Tukey’s multiple comparison test. Statistical significance is indicated as follows: ** P < 0.01; **** P < 0.0001. Representative FACS plots were pre-gated based on the following parameters: Singlets, Live, Lymphocytes, CD45 + , CD3/5 + , CD4 + CD44 + , CD62L lo . Frequency of parental gate +/− standard error of the mean is shown in green. Cells were stimulated with peptide or DMSO for 1 hour before the addition of BFA-M. Cells were incubated for an additional 4 hours before intracellular cytokine staining and analysis by flow cytometry.

Journal: bioRxiv

Article Title: Generation and characterization of a novel MHC-II tetramer for tracking and characterization of toxin B-specific CD4 + T cell responses

doi: 10.64898/2026.02.18.706639

Figure Lengend Snippet: TcdB 1918 and TcdB 1961 are immunodominant epitopes following vaccination. (A) Amino acid sequences from TcdB peptide library . 15mers were analyzed using the Immune epitope database MHC-II peptide binding prediction for H2-IAb. Data were represented as adjusted rank which is generated by comparing each peptide’s score to a database of 15mers. Lower numbers are predicted to be better binders to H2-IAb. (B) Representative FACS plots showing frequency of TcdB pooled peptide library, individual peptides, or DMSO stimulated CD4 + T cells expressing IFN-γ. (C) The total number of IFN-γ + CD4 + T cells responding to TcdB peptide library or individual peptides. To account for the background cytokine production observed, the total number of IFN-γ + CD4 + T cells in the DMSO group was subtracted from the total number of peptide-responsive IFN-γ + CD4 + T cells. Naïve n=5 and TcdB-CROPs mRNA vaccinated n=5 per timepoint. Two-way ANOVA with Tukey’s multiple comparison test. Statistical significance is indicated as follows: ** P < 0.01; **** P < 0.0001. Representative FACS plots were pre-gated based on the following parameters: Singlets, Live, Lymphocytes, CD45 + , CD3/5 + , CD4 + CD44 + , CD62L lo . Frequency of parental gate +/− standard error of the mean is shown in green. Cells were stimulated with peptide or DMSO for 1 hour before the addition of BFA-M. Cells were incubated for an additional 4 hours before intracellular cytokine staining and analysis by flow cytometry.

Article Snippet: Potential immunodominant epitopes in our TcdB peptide library were identified using predicted immunogenicity analysis from the immune epitope database (IEDB) , on the TcdB-CROPs peptide library generated previously .

Techniques: Binding Assay, Generated, Expressing, Comparison, Incubation, Staining, Flow Cytometry